Zero-cost modification of bright field microscopes for imaging phase gradient on cells: Schlieren optics
- 1 June 1981
- journal article
- Published by Springer Nature in Cell Biochemistry and Biophysics
- Vol. 3 (2) , 167-173
- https://doi.org/10.1007/bf02788132
Abstract
A simple, zero-cost, reversible modification of a bright field microscope permits visualization of phase gradients in cells by transmitted illumination, yielding a Nomarski-like effect. This modification, based on schlieren optics, is simultaneously compatible with high-aperture epi-illumination fluorescence excitation. For many objectives that are intended for use in fluorescence work, but are unavailable in phase contrast versions, the modification provides a simple means for locating cells in culture with good image contrast and resolution.Keywords
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