Limits to the role of palindromy in deletion formation
- 1 January 1991
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 173 (1) , 315-318
- https://doi.org/10.1128/jb.173.1.315-318.1991
Abstract
We tested the effect of palindromy on deletion formation. This involved a study of reversion of insertion mutations in the pBR322 amp gene at a site where deletions end either in 9-bp direct repeats or in adjoining 4-bp direct repeats. Inserts of palindromic DNAs ranging from 10 to more than 26 bp and related nonpalindromic DNAs were compared. The frequency of deletions (selected as Ampr revertants) was stimulated by palindromy only at lengths greater than 26 bp. The 4-bp direct repeats, one component of which is located in the palindromic insert, were used preferentially as deletion endpoints with palindromes of at least 18 bp but not of 16 or 10 bp. We interpret these results with a model of slippage during DNA replication. Because deletion frequency and deletion endpoint location depend differently on palindrome length, we propose that different factors commit a molecule to undergo deletion and determine exactly where deletion endpoints will be.Keywords
This publication has 10 references indexed in Scilit:
- Sequence specificity of pausing by DNA polymerasesBiochemical and Biophysical Research Communications, 1989
- Sequence-specific pausing during in vitro DNA replication on double-stranded DNA templatesJournal of Biological Chemistry, 1989
- Specificity of deletion events in pBR322Plasmid, 1989
- Mechanisms of spontaneous mutagenesis: An analysis of the spectrum of spontaneous mutation in the Escherichia coli lacI geneJournal of Molecular Biology, 1986
- IS50-mediated inverse transposition: specificity and precisionGene, 1985
- Structural intermediates of deletion mutagenesis: a role for palindromic DNA.Proceedings of the National Academy of Sciences, 1984
- On the formation of spontaneous deletions: The importance of short sequence homologies in the generation of large deletionsCell, 1982
- Analysis of gene control signals by DNA fusion and cloning in Escherichia coliJournal of Molecular Biology, 1980
- Genetic studies of the lac repressorJournal of Molecular Biology, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977