Phosphorylation of the 90 kDa heat shock protein in heat shocked HeLa cell lysates
- 25 April 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 231 (2) , 417-420
- https://doi.org/10.1016/0014-5793(88)80862-7
Abstract
The 90 kDa heat shock protein (hsp 90) is a major phosphorylated protein under normal growth conditions. However, it does not incorporate detectable levels of phosphate by incubation of control HeLa cell lysates with [γ-32P]ATP in vitro. In this paper we show that strong phosphorylation of hsp 90 occurs in lysates prepared from heat shocked HeLa cells. Possible involvement of the eukaryotic initiation factor 2 kinase of the heme-controlled repressor of translation is discussed.Keywords
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