A novel δ° arising from a frameshift insertion, detected by direct sequencing of enzymatically amplified DNA
- 1 August 1989
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 83 (1) , 75-78
- https://doi.org/10.1007/bf00274153
Abstract
We describe a novel mutation in the δ globin gene of a compound heterozygote for δ° thalassemia and a deletion type Gγ+(Aγδβ)° thalassemia. The δ gene was amplified using the polymerase chain reaction (PCR), and the amplified material was used in a direct sequencing experiment. The nucleotide sequence of the mutant δ gene showed that the insertion of an extra nucleotide at the third position of codon 91 in the second exon, which gives rise to a premature stop codon at position 94, leads to the silencing of this gene. The presence of the mutation in the carriers of δ-thalassemia in this family was confirmed by dot blot hybridization. A possible model for the insertion of the extra nucleotide is discussed.This publication has 14 references indexed in Scilit:
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