Separation and Purification of Hepatitis-associated Antigen into Morphologic Types by Zonal Ultracentrifugation

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Abstract
Partially purified hepatitis-associated antigen derived from preliminary isopycnic banding in cesium chloride was further purified and separated into its various types of particles by sedimentation-velocity techniques. This was done in a zonal ultracentrifuge rotor with cesium-chloride gradients. Three morphologic types of particles were found; all three appear to be similar by chemical and immunologic criteria. The complexity of the particles evidenced by the diverse morphologic types suggests that the term hepatitis-associated antigen is inadequate to describe the structures.