The Use of 2,3,5-Triphenyl-Tetrazoliumchloride as a Measure of Seed Germinability

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Abstract
Comparative determinations of seed viability by germination tests and by staining indicate that the latter method holds promise as a rapid means of obtaining an estimate of germinability. The staining method involves soaking and sectioning the seed through the embryo before placing in the staining solution. Seeds used in the investigation included primarily members of the grass family and some legumes. In the grasses, a good correlation between staining and actual germination was obtained in several cases. Less favorable results were obtained with the legumes because of difficulties in sectioning and failure in detecting certain seedling abnormalities. The accuracy of the staining method in determining the viability of dormant seeds was not investigated. The stain is a colorless substance which forms a red compound on reduction. Seed viability is indicated by the presence or absence or localization of coloration in the embryo.

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