Molecular cloning and expression of a locus (mdoA) implicated in the biosynthesis of membrane‐derived oligosaccharides in Escherichia coli
- 1 September 1989
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 3 (9) , 1173-1182
- https://doi.org/10.1111/j.1365-2958.1989.tb00267.x
Abstract
Summary: Mutants of Escherichia coli defective in the mdoA locus are blocked at an early stage in the biosynthesis of membrane‐derived oligosaccharides. The mdoA locus has now been cloned into multicopy plasmids. A 5kb DNA fragment is necessary to complement mdoA mutations. Cells harbouring the mdoA+ plasmid produced three to four times more MDO than wild‐type cells. MDO overproduction did not affect the degree of MDO substitution with sn‐1‐phosphoglycerol residues. The biosynthesis of MDO remained under osmotic control in overproducing strains.This publication has 27 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- The function of acyl carrier protein in the synthesis of membrane-derived oligosaccharides does not require its phosphopantetheine prosthetic group.Proceedings of the National Academy of Sciences, 1987
- Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9Gene, 1986
- New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transpositionGene, 1984
- Studies on the structure and expression of Escherichia coli pyrC, pyrD and pyrF using the cloned genesEuropean Journal of Biochemistry, 1984
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Osmotic regulation and the biosynthesis of membrane-derived oligosaccharides in Escherichia coli.Proceedings of the National Academy of Sciences, 1982
- Prolonged incubation in calcium chloride improves the competence of Escherichia coli cellsGene, 1979
- A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genomeCell, 1976
- [1] Analysis of sugars found in glycoproteinsPublished by Elsevier ,1966