ANDROGRAPHOLIDE AND KALMEGH (ANDROGRAPHIS-PANICULATA) EXTRACT - INVIVO AND INVITRO EFFECT ON HEPATIC LIPID-PEROXIDATION

  • 1 January 1984
    • journal article
    • research article
    • Vol. 6  (9) , 481-485
Abstract
Single or repeated (for 15 consecutive days) oral administration of kalmegh leaf extract (500 mg/kg) or its bitter principle, andrographolide (5 mg/kg) to adult made albino rats (body wt 125-150 g) produced no significant change in NADPH induced hepatic microsomal lipid peroxidation. CCl4 (5 ml/kg), induced microsomal lipid peroxidation was decreased when the rats were pretreated (for 4 h), but only with a single dose and not with long-term administration of kalmegh or andrographolide. In vitro CCl4 (1 .mu.l) induced hepatic microsomal lipid peroxidation was completely normalized by kalmegh leaf extract (0.5 and 5.0 .mu.g/mg protein) or andrographolide (0.5 and 5.0 .mu.g/mg protein). At the higher concentration of CCl4 (2 .mu.l), hepatic microsomal lipid peroxidation remained significantly increased (25%) in the presence of andrographolide (0.5 .mu.g/mg protein), but not in the presence of kalmegh extract (0.5 .mu.g/protein). Kalmegh leaf extract apparently has more protective action on CCl4 induced hepatic toxicity than its bitter principle, andrographolide.