Genome Plasticity among Related Lactococcus Strains: Identification of Genetic Events Associated with Macrorestriction Polymorphisms
- 1 May 2000
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 182 (9) , 2481-2491
- https://doi.org/10.1128/jb.182.9.2481-2491.2000
Abstract
The genomic diversity of nine strains of the Lactococcus lactis subsp. cremoris (NCDO712, NCDO505, NCDO2031, NCDO763, MMS36, C2, LM0230, LM2301, and MG1363) was studied by macrorestriction enzyme analysis using pulsed-field gel electrophoresis. These strains were considered adequate for the investigation of genomic plasticity because they have been described as belonging to the same genetic lineage. Comparison of Apa I and Sma I genome fingerprints of each strain revealed the presence of several macrorestriction fragment length polymorphisms (RFLPs), despite a high degree of similarity of the generated restriction patterns. The physical map of the MG1363 chromosome was used to establish a genome map of the other strains and allocate the RFLPs to five regions. Southern hybridization analysis correlated the polymorphic regions with genetic events such as chromosomal inversion, integration of prophage DNA, and location of the transposon-like structures carrying conjugative factor or oligopeptide transport system.Keywords
This publication has 79 references indexed in Scilit:
- Large genome rearrangements discovered by the detailed analysis of 21 Pseudomonas aeruginosa clone C isolates found in environment and disease habitats 1 1Edited by J. KarnJournal of Molecular Biology, 1997
- Analysis of the genetic polymorphism between three Streptococcus thermophilus strains by comparing their physical and genetic organizationMicrobiology, 1997
- The unstable region of Streptomyces ambofaciens includes 210 kb terminal inverted repeats flanking the extremities of the linear chromosomal DNAMolecular Microbiology, 1996
- Transport of β-Casein-derived Peptides by the Oligopeptide Transport System Is a Crucial Step in the Proteolytic Pathway of Lactococcus lactisPublished by Elsevier ,1995
- Conservation of gene arrangement and an unusual organization of rRNA genes in the linear chromosomes of the Lyme disease spirochaetes Borrelia burgdorferi, B. garinii and B. afzeliiMicrobiology, 1994
- Bacterial genomicsFEMS Microbiology Reviews, 1994
- Relationship between Lactococcus lactis subsp. lactis 952, an industrial lactococcal starter culture and strains 712, ML3 and C2Journal of Applied Bacteriology, 1993
- XbaI and BlnI Genomic Cleavage Maps of Escherichia coli K-12 Strain MG1655 and Comparative Analysis of Other StrainsJournal of Molecular Biology, 1993
- Cleavage pattern of the homing endonuclease encoded by the fifth intron in the chloroplast large subunit rRNA-encoding gene of Chlamydomonas eugametosGene, 1991
- The Value of Plasmid Profiles for Strain Identification in Lactic Streptococci and the Relationship between Streptoccocus lactis 712, ML3 and C2Journal of Applied Bacteriology, 1981