The quantitation of biotinylated compounds by a solid‐phase assay using a 125I‐labelled biotin derivative
- 11 May 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 215 (2) , 305-310
- https://doi.org/10.1016/0014-5793(87)80167-9
Abstract
The biotin analogue biotinylglycyltyrosine has been synthesized and labelled to a specific activity of 2000 Ci/mmol with 125I. This analogue has been used in conjunction with immobilized streptavidin in an assay which detects as little as 1 fmol biotin or biotinylated molecules in solution. The determination of biotinylated insulin in a tissue extract and the quantitation of a transcription assay are given as examplesKeywords
This publication has 11 references indexed in Scilit:
- Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.Proceedings of the National Academy of Sciences, 1983
- Solid-phase assay for d-biotin on avidin-cellulose disksAnalytical Biochemistry, 1981
- Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.Proceedings of the National Academy of Sciences, 1981
- Interaction of the activated cytoplasmic glucocorticoid hormone receptor complex with the nuclear envelopeBiochemistry, 1981
- [51] A rapid, sensitive fluorometric assay for avidin and biotinPublished by Elsevier ,1979
- [50] Isotope dilution assay for biotin: Use of [3H]biotinPublished by Elsevier ,1979
- [49] Isotopic dilution assay for biotin: Use of [14C]biotinPublished by Elsevier ,1979
- Use of avidin-sepharose to lsolate and identify biotin polypeptides from crude extractsAnalytical Biochemistry, 1978
- A protein-binding assay for measurement of biotin in physiological fluidsClinica Chimica Acta; International Journal of Clinical Chemistry, 1978
- Biotinylinsulins as potential tools for receptor studiesProceedings of the National Academy of Sciences, 1977