Screening of Chloramphenicol Residues in Pork Muscle by Planar Chromatography
- 1 September 1994
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 77 (5) , 1101-1104
- https://doi.org/10.1093/jaoac/77.5.1101
Abstract
A rapid planar chromatographic method is described for the qualitative determination of chloramphenicol residues in muscle. The drug is extracted with ethyl acetate and purified by solid-phase extraction. After elution, the collected phase is evaporated to dryness. The residue is dissolved in methanol, spotted on a silica gel plate, and chromato-graphed. After elution, the chloramphenicol is visualized after reduction with fluorescamine. This method detects chloramphenicol in meat at 10 μg/kg and has a sample throughput of about 25 samples per analyst per day.Keywords
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