Axial ligand replacement in horse heart cytochrome c by semisynthesis
- 1 January 1989
- journal article
- research article
- Published by Wiley in Proteins-Structure Function and Bioinformatics
- Vol. 6 (3) , 338-340
- https://doi.org/10.1002/prot.340060316
Abstract
Semisynthesis has been employed to replace the axial methionine in horse heart cytochrome c with histidine. The reduction potential of the His-80 protein (cyt c-His-80) is 41 mV vs NHE (0.1 M phosphate; pH 7.0; 25°C). The absorption spectra of oxidized and reduced cyt c-His-80 are very similar to those of the native protein in the porphyrin region, but the 695 nm band is absent in the oxidized His-80 protein.Keywords
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