The development of a monoclonal antibody to a human gamma crystallin

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Abstract
A monoclonal antibody to human lens 24,000 MW .gamma.-crystallin was produced in rat .times. mouse hybridoma cell lines. The antibody reacts specifically with the 24,000 MW .gamma.-crystallin and does not cross react with the 21,000 or 19,000 .gamma.-crystallins. The antibody reacts with protein from dog, mouse, monkey and bovine lenses, but does not react with proteins from chick lens. The 24,000 MW .gamma. protein increases from 2% of the soluble protein in the fetal lens to 14% in the 2 mo. old lens to 25% in the adult lens. No difference was observed in the amount of this crystallin between the nuclear and cortical regions of the adult lens. In 1 hypermature cataractous lens, the amount of this crystallin decreased to a value of .apprx. 5% of the total soluble protein.