Cloning, Nucleotide Sequence, and Expression of the Brucella melitensis sucB Gene Coding for an Immunogenic Dihydrolipoamide Succinyltransferase Homologous Protein
- 1 October 2001
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 69 (10) , 6537-6540
- https://doi.org/10.1128/iai.69.10.6537-6540.2001
Abstract
The Brucella melitensis sucB gene encoding the dihydrolipoamide succinyltransferase (E2o) enzyme (previously identified as an immunogenic protein in infected sheep) was cloned and sequenced. The amino acid sequence predicted from the cloned gene revealed 88.8 and 51.2% identity to the dihydrolipoamide succinyltransferase SucB protein from Brucella abortus and Escherichia coli , respectively. Sera from naturally infected sheep showed antibody reactivity against the recombinant SucB protein.Keywords
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