A Simple Method for the Preparation of Phosphatidylcholine Labelled at 2-Acyl Position
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 11 (1) , 91-97
- https://doi.org/10.1080/00327488108068727
Abstract
Phosphatidylcholine with a labelled acyl group at 2 position was prepared enzymatically from lysophosphatidylcholine and labelled fatty acids. Under optimal conditions, over 90% of [5,6,8,9,11,12,14,15-3H] arachidonic acid, [9,10-3H] palmitic acid, [1-14C] linoleic acid or [9,10-3H] oleic acid was incorporated into the phosphatidylcholine, and the incorporation was quantitative up to 500 nmol of [1-14C] linoleic acid. Analysis of the labeled phosphatidylcholine formed indicated that radioactivity was incorporated exclusively at 2-acyl position.This publication has 12 references indexed in Scilit:
- Synthesis of 1-palmitoyl and 1-stearoyl phosphatidylcholines from mixtures of acyl acceptors via acyl-CoA:1-Acyl-sn-glycero-3-phosphorylcholine acyltransferase in liver microsomesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1979
- Preparation of acylglycerols and phospholipids with the aid of lipolytic enzymesJournal of Oil & Fat Industries, 1978
- ON THE SITE OF ACTION OF PHOSPHATIDE ACYL‐HYDROLASE ACTIVITY OF RAT BRAIN HOMOGENATES ON LECITHINJournal of Neurochemistry, 1968
- Purification and properties of phospholipase A1 from rat and calf brainBiochimica et Biophysica Acta (BBA) - Enzymology, 1968
- Hydrolysis of phospholipids and glycerides by rat-liver preparationsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1967
- Synthesis of 32P-, 14C-, and 3H-Labeled Lecithins and Their Use in Studies on Lipid MetabolismPublished by Springer Nature ,1966
- Positional Specificities in Phospholipid Hydrolyses*Biochemistry, 1962