Isohormones of Human Growth Hormone. II. Plasmin-Catalyzed Transformation and Increase in Prolactin Biological Activity*
- 1 October 1973
- journal article
- other
- Published by The Endocrine Society in Endocrinology
- Vol. 93 (4) , 858-865
- https://doi.org/10.1210/endo-93-4-858
Abstract
Digestion of human growth hormone (hGH) with the proteolytic enzyme, plasmin, leads to a progressive transformation of isohormone B to isohormones C, D and E. This transformation was followed by polyacrylamide gel electrophoresis (PAGE) at pH71π 7.8, 0 C as a progressive increase in intensity of bands of higher mobility, and a corresponding diminution of bands of lower mobility. This band transformation was paralleled by an increase of prolactin biological specific activity. Digestion was not accompanied by deamidation of hGH. (Endocrinology93: 858, 1973)Keywords
This publication has 0 references indexed in Scilit: