Identification of an RFLP marker tightly linked to theHt1 gene in maize
- 1 January 1991
- journal article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 82 (4) , 393-398
- https://doi.org/10.1007/bf00588588
Abstract
We have identified tight linkage of an RFLP marker to theHt1 gene of maize that confers resistance to the fungal pathogenHelminthosporium turcicum race 1. This was accomplished by the use of four pairs of near isogenic lines (NILs; B73, A619, W153R, and CM105), each differing by the presence or the absence of the geneHt1. SinceHt1 maps to chromosome 2, 26 clones already mapped to this chromosome were labeled and probed against Southern blots of these NILs DNA digested with three restriction enzymes:EcoRI,BamHI, andHindIII. Six markers exhibited an RFLP for at least one pair of NILs. Presumptive linkage was further tested by analyzing the segregation of five of the six markers (one was monomorphic in the cross studied) and resistance toH. turcicum race 1 on 95 F2 individuals from the cross DF20 × LH146Ht. The results indicate a tight linkage between one of the DNA markers,UMC150B, and theHt1 gene.Keywords
This publication has 15 references indexed in Scilit:
- RFLP analysis of the size of chromosomal segments retained around the Tm-2 locus of tomato during backcross breedingTheoretical and Applied Genetics, 1989
- Approaches to Cloning Plant Genes Conferring Resistance to Fungal PathogensAnnual Review of Phytopathology, 1988
- MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populationsGenomics, 1987
- Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphismsTheoretical and Applied Genetics, 1986
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- Reactions of Eighty-Four Sources of Chlorotic Lesion Resistance in Corn to Three Biotypes ofHelminthosporium turcicumPlant Disease, 1981
- A Second Major Gene Locus in Corn for Chlorotic‐Lesion Resistance to Helminthosporium turicum1Crop Science, 1977
- Gene Action Conditioning Resistance to Northern Leaf Blight in Maize1Crop Science, 1971
- Monogenic Resistance in Zea mays L. to Helminthosporium turcicum1Crop Science, 1963
- Inheritance of Resistance to the Leaf Blight of Corn Caused by Helminthosporium turcicumAgronomy Journal, 1952