HumanADAM33Messenger RNA Expression Profile and Post-Transcriptional Regulation

Publisher Website
Google Scholar
Abstract
We examined transcript expression and post-transcriptional regulation of human ADAM33, a recently identified asthma gene. A detailed messenger RNA (mRNA) expression profile was obtained using Northern, reverse transcription polymerase chain reaction, and in situ hybridization analyses. ADAM33 mRNA was expressed significantly in smooth muscle–containing organs, minimally in immune organs and hematopoietic cells, and highly in repairing duodenal granulation tissue. Expression was seen in asthmatic subepithelial fibroblasts and smooth muscle but not in respiratory epithelium. In all tissues, transcripts of ∼ 5 kb predominated over those of ∼ 3.5 kb by 2- to 5-fold. The effect of the 3′ untranslated region (UTR) on ADAM33 protein expression and maturation was examined. The presence of the 3′UTR in untagged full-length constructs promoted prodomain removal, detected as mature ∼ 100 kD protein by ADAM33-reactive antibodies; in its absence, maturation was 2- to 3-fold less in HEK293 cells. His-tagged and untagge...