AN ASSAY FOR DETERMINING THE RELATIVE AFFINITY OF TRACE AMOUNTS OF LABELED ANTIBODIES

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Abstract
An assay for the binding efficiency or "relative affinity" of trace amounts of radioactively labeled anti-2,4-dinitrophenyl (DNP) antibodies was developed. The assay measures the relative ability of the labeled antibodies to combine and precipitate with antigen in the presence of a large amount of unlabeled reference antibody of the same specificity. The relative affinity of the labeled antibodies for dinitrophenylated antigens was correlated with the association constants of the reference antibodies for simple univalent DNP ligands.