Identification of the ligand trans to thiolate in cytochrome P‐450 LM2 by chemical modification
- 8 August 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 159 (1-2) , 58-62
- https://doi.org/10.1016/0014-5793(83)80416-5
Abstract
About 3 tyrosine residues of cytochrome P‐450 LM2 are accessible to chemical modification with tetranitromethane. Nitration of two tyrosines inactivates the enzyme to about 20%. The partial formation of a hyper‐porphyrin spectrum originating from the pK shift by nitration and formation of a tyrosinate is prevented by modification in the presence of the inhibitor metyrapone. These findings support the assumption of a tyrosine residue as sixth ligand of the heme iron in cytochrome P‐450 LM2.Keywords
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