CELLULAR AND MOLECULAR HETEROGENEITY OF H‐2Kk ANTIGENS

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Abstract
Up to 70% of mouse spleen cells expressing H-2Kk alloantigens reacted with the monoclonal anti-H-2Kk antibody 11-4.1 in an indirect rosetting assay. When the cells that rosetted with the monoclonal antibody 11-4.1 were removed by density centrifugation, the residual unreactive cells reacted with the polyclonal anti-H-2Kk alloantiserum D23, but not with the monoclonal antibody 11-4.1; this indicated the existence of 2 cell populations. In sequential immunoprecipitation experiments the glycoprotein fraction of NP40 extracts from H-2Kk cells, after removal of antigens reacting with the monoclonal antibody 11-4.1, still contained structures reactive with the alloantiserum. Thus, there are at least 2 antigenically distinct H-2Kk molecules which are differentially expressed on 2 subpopulations of spleen cells.