Metabolic phases during the development of granulation tissue

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Abstract
The metabolism of incubated slices of sponge-induced granulation tissue, harvested 4-90 days after the implantation, was studied with special reference to the capacity of collagen synthesis and to the energy metabolism. Data are also given on the nucleic acid contents during the observation period. Three metabolic phases were evident. The viability of the slices for the synthesis of collagen was studied in various conditions. Freezing and homo-genization destroyed the capacity of the tissue to incorporate pro-line into collagen. Consumption of O2 reached the maximum at 30-40 days. There was evidence that the pentose phosphate cycle was important, especially during the phases of the proliferation and the involution. The formation of lactic acid was maximal at about 20 days. The capacity to incorporate proline into collagen hydroxy-proline in vitro was limited to a relatively short period at 10-30 days. The synthesis of collagen was dependent on the supply of O2 and glucose, the latter could be replaced in the incubation medium by other monosaccharides but not by the metabolites of glucose or tricarboxylic acid-cycle intermediates.

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