In vivo sequence requirements of the SV40 early promoter region

Abstract
To investigate the sequences necessary for proper initiation of transcription of SV40 early genes, several mutants with deletions in the promoter region were constructed. The adenine-thymidine-rich TATA box region is apparently involved in fixing initiation precisely within a narrow area, but is dispensable for gene expression; sequences located > 150 base pairs upstream are indispensable for gene expression.