A DIFFUSION METHOD FOR THE ESTIMATION OF FORMALDEHYDOGENIC STEROID*

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Abstract
ONE method for the estimation of cortical hormone in urine is the determination of the formaldehyde produced by the oxidation of the ketol side chain with periodic acid (Lowenstein, Corcoran and Page, 1946). In order to minimize the contribution of non-specific organic material, Daughaday, Jaffee and Williams (1948) developed a method in which the formaldehyde is distilled before it is estimated by means of the color reaction with chromotropic acid. Corcoran and Page (1948) have developed similar procedures. It occurred to us that isothermal distillation of the formaldehyde by means of Conway cells (Conway, 1947) would be useful in this determination. One such method has been reported by Bassil and Hain (1950) but is inconvenient since it requires three to four days to reach equilibrium and does not permit quantitative recovery. The solution of this problem is to allow the distillation to occur directly into the chromotropic acid reagent, which is dissolved in 15 M

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