5-Enolpyruvylshikimate 3-Phosphate Synthase, the Target Enzyme of the Herbicide Glyphosate, Is Synthesized as a Precursor in a Higher Plant
- 1 February 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 83 (2) , 229-231
- https://doi.org/10.1104/pp.83.2.229
Abstract
Cell cultures of Corydalis sempervirens adapted to growth in the presence of 5 millimolar glyphosate overproduce the herbicide''s target enzyme, 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, 30- to 40-fold. In vitro translation of total RNA and poly(A)-RNA coupled with immunoprecipitation showed that the protein is synthesized as a precursor of relative molecular weight (Mr) 53,900 .+-. 900 as compared to Mr 45,500 .+-. 1000 of the mature enzyme. Translatable activity of mRNA for EPSP-synthase in glyphosphate-adapted cultures is tenfold higher than in nonadapted cultures.Keywords
This publication has 21 references indexed in Scilit:
- Ornithine decarboxylase inhibitors increase the cellular content of the enzyme: Implications for translational regulationBiochemical and Biophysical Research Communications, 1985
- Selection and Characterization of a Carrot Cell Line Tolerant to GlyphosatePlant Physiology, 1984
- 5‐Enolpyruvylshikimate‐3‐phosphate synthase of Klebsiella pneumoniaeEuropean Journal of Biochemistry, 1984
- GENE AMPLIFICATIONAnnual Review of Biochemistry, 1984
- Kinetics of 5‐enolpyruvylshikimate‐3‐phosphate synthase inhibition by glyphosateFEBS Letters, 1983
- Biosynthetic pathways of two polypeptide subunits of the light-harvesting chlorophyll a/b protein complex.The Journal of cell biology, 1981
- The isolation and characterization of Drosophila yolk protein genesCell, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- An Efficient mRNA‐Dependent Translation System from Reticulocyte LysatesEuropean Journal of Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970