MICROCALORIMETRIC STUDY OF GROWTH OF KLEBSIELLA-AEROGENES IN SIMPLE SALTS-GLUCOSE MEDIA

  • 1 January 1976
    • journal article
    • research article
    • Vol. 16  (63) , 37-48
Abstract
Heat output-time records or thermograms produced during the aerobic growth of K. aerogenes in simple salts/glucose media with growth limiting glucose concentrations of 2.0, 1.0 and 0.5 g dm-3 were obtained using a flow microcalorimeter fitted with an aerobic cell. These traces are interpreted in terms of the recorded O2 tension, pH, glucose concentration and bacterial population of the culture. Heat output is greatest during the phase of exponential growth, indicating that here the organisms are most energetically inefficient. During the stationary phase aerobic processes, which give rise to a low O2 tension, produce a smaller heat output until secondary metabolic processes are complete.

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