Abstract
G-6-P dehydrogenase activity in rat liver mitochondria can be released by detergent. The released activity is separated by chromatography into 2 peaks. One peak has the kinetic behavior and mobility similar to the soluble sex-linked enzyme, whereas the other peak is similar to the microsomal hexose 6-phosphate dehydrogenase. There is no evidence for the existence of a new G-6-P dehydrogenase activity in rat liver mitochondria.