Specificities and some properties of human T and B lymphocyte antisera.

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Abstract
Antisera were prepared in rabbits by immunization with either human peripheral blood T lymphocytes (PTL) or tonsil B lymphocytes (TBL). Specificites of sequentially absorbed antisera were examined by cytotoxicity testing, immunoperoxidase staining and absorption studies. Anti-PTL serum (ATS) reacted with E [erythrocyte] rosetting lymphocytes in the normal population. ATS was also reactive with some of lymphoid neoplasms, whether neoplastic cells carried E receptors or not. This serum did not lyse any of myelogenous leukemia cells. When antiserum was used in the immunoperoxidase staining, ATS strongly stained paracortical cells in the lymph node and medullary thymocytes in the thymus. Anti-TBL serum (ABS) was reactive with lymphocytes corresponding to the percentage of erythrocyte-antibody-complement complex binding lymphocytes in the normal population. ABS reacted not only with the neoplastic cells carrying complement component 3 receptors and/or surface Ig, but also with non-T non-B acute lymphoblastic leukemia cells, and a small number of acute myelogenous leukemia cells. In the immunoperoxidase staining, lymph follicular cells showed positive immunoreactivity with ABS, but paracortical cells did not.