Abstract
The 5'' terminus of the rat preproinsulin II gene exhibits a tissue-dependent DNase I sensitivity. Only in the chromatin from a pancreatic beta-cell tumor, but not in liver, spleen, kidney, or brain chromatin, is a region at and before the 5'' end of the gene exposed to cleavage. The region of exposure extends 250-300 base-pairs upstream from the 5'' terminus of the preproinsulin mRNA. Such a region may allow control sequences special access to regulatory proteins.