Alternative sites of transcription initiation upstream of the canonical cap site in human -γ-globin andβ-globin genes
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (4) , 1811-1820
- https://doi.org/10.1093/nar/12.4.1811
Abstract
Using S1 mapping and primer extension analysis, we have identified a number of human kappa-globin and beta-globin 5' RNA termini originating in the 200 bp upstream of the canonical mRNA cap sites. Upstream initiation sites have previously been reported for the human epsilon-globin gene (4,5) and the present work indicates that this is a general feature of the human beta-type globin genes. We have attempted to identify features common to such sites between the three genes. One site 170 bp upstream of the major beta-globin cap site and a site 1400 bp upstream of the major epsilon-globin cap site are located near putative PolIII promoter sequences and may therefore be transcribed by this enzyme. Alternative initiation sites located 200 bp and 50-100 bp upstream of the epsilon-globin and kappa-globin cap sites respectively are located within S1 hypersensitive regions of chromatin.Keywords
This publication has 20 references indexed in Scilit:
- Multiple origins of transcription in the 4.5 Kb upstream of the ϵ-globin geneCell, 1983
- Human β-globin promoter and coding sequences transcribed by RNA polymerase IIICell, 1983
- Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit β-globin gene in mouse 3T6 cellsCell, 1983
- Fine structure of the origin-proximal DNAase I-hypersensitive region in wild-type and EC mutant polyomaCell, 1981
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Heterogeneity and 5′-terminal structures of the late RNAs of simian virus 40Journal of Molecular Biology, 1978
- Complete nucleotide sequence of the 5′ noncoding region of human α- and β-globin mRNACell, 1977
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Globin RNA precursor molecules: Biosynthesis and processing in erythroid cellsCell, 1977
- A precursor of globin messenger RNAJournal of Molecular Biology, 1976