Replacement of wybutine by hydrazines and its effect on the active conformation of yeast tRNAPhe
- 1 January 1978
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 5 (5) , 1701-1713
- https://doi.org/10.1093/nar/5.5.1701
Abstract
The highly modified base wybutine (YWye) next to the anticodon of yeast tRNAPhe has been replaced by different hydrazine derivatives. The effect of the replacement on the activity of the tRNA has been studied in the heterologous aminoacylation with synthetase from E. coli and in the poly(U) directed binding to ribosomes from both yeast and E. coli. It was found that starting from tRNA-PheYWye the activity increased with increasing size, aromaticity, and stacking tendency of the substituent replacing YWye. It is concluded that YWye by the size of its aromatic system and by its stacking properties is particularly well suited for stabilizing the native conformation of tRNAPhe.Keywords
This publication has 27 references indexed in Scilit:
- Structural Domains of Transfer RNA MoleculesScience, 1976
- Tertiary structure interactions of 7‐methylguanosine in yeast tRNAPhe as studied by borohydride reductionFEBS Letters, 1975
- Efficient polyphenylalanine synthesis with proflavine and ethidium labeled tRNAPhe from yeast in the reticulocyte ribosomal systemBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- New fluorescent hydrazide reagents for the oxidized 3'-terminus of RNANucleic Acids Research, 1974
- l‐Phenylalanyl‐tRNA Synthetase of Escherichia coli K‐10European Journal of Biochemistry, 1974
- Incorporation of 3‐Methyl‐2‐benzothiazolone Hydrazone into the Anticodon Loop of tRNAPhe from YeastEuropean Journal of Biochemistry, 1972
- Replacement of Y base, dihydrouracil, and 7‐methylguanine in tRNA by artificial odd basesFEBS Letters, 1971
- Study on the properties and structure of the modified base Y+ of yeast tRNAPheBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- STUDIES ON POLYNUCLEOTIDES .80. YEAST PHENYLALANINE TRANSFER RIBONUCLEIC ACID - PRODUCTS OBTAINED BY DEGRADATION WITH RIBONUCLEASE T1968
- RNA Codewords and Protein SynthesisScience, 1964