SOME PROPERTIES OF AN IMMUNE FACTOR ISOLATED FROM THE BLOOD OF ACTIVELY IMMUNIZED WAX MOTH LARVAE

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Abstract
The blood of wax moth larvae immunized against Pseudomonas aeruginosa contains a fraction that is bactericidal to P. aeruginosa, confers immunity, and inhibits melanization; this fraction was isolated by dialysis, by precipitation with, ethanol, and by absorption on an anion exchange resin. When immune blood was dialyzed it darkened like normal blood, and lost its bactericidal activity and protective power. Material removed by dialysis and freeze-dried showed bactericidal activity and protective power in some experiments but not in others. Ethanol fractionation of immune blood gave a precipitate that was bactericidal to P. aeruginosa, passively protected normal larvae, and inhibited tyrosinase activity. Absorption treatment of immune blood with an anion exchange resin followed by elution with sodium hydroxide produced material with similar properties. The factor was dialyzable, heat stable, unaffected by trypsin, weakly acidic, and of relatively small molecular weight.

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