Growth and Acid Production by Oral Lactobacilli in the Presence of Varying Amounts of Required Vitamins

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Abstract
Vitamins needed for the growth of oral lactobacilli were each added in graded amts. to a basal medium of casein digest, glucose, and salts. When the amt. of one needed vitamin was reduced, ample supplies of other needed vitamins and nutritive substances were present. In general, the following amts. of vitamins sufficed for maximal or practically maximal growth and acid production (about pH 4): in terms of [mu]g./ml. of medium, biotin 0.0003, niacin 0.05, Ca pantothenate 0.03-0.1, riboflavin 0.03, pteroylglutamic acid about 0.01, pyridoxamine hydrochloride 0.3-1.0, pyridoxal hydrochloride 0.03-0.3, and thiamine hydrochloride 0.01-0.03. Smaller amts. of vitamins supported less growth and acid production. With allowance for individual variations, amounts of each vitamin approx. 10% of those given above sufficed for attainment of about pH 5.