Notes on the preparation and assay of anti-lymphocytic serum for use in mice.
- 1 November 1968
- journal article
- Vol. 15 (5) , 697-705
Abstract
The `activity' of rabbit anti-mouse ALS was measured in terms of its power to prolong the life of A-strain homografts on CBA mice. Antisera raised by lymphocytes from mice of other strains were active in CBA mice, and conversely antisera raised by CBA lymphocytes were active in mice of other strains. All active sera were cytotoxic to mouse lymphocytes in the presence of mouse complement, but not all cytotoxic sera were active. ALS raised in species (chicken, duck) of which the sera do not bind with mouse complement were inactive in mice. Neither cytotoxicity nor the power to circumvent graft-versus-host reactions give adequate measures of the activity of impure antisera.This publication has 18 references indexed in Scilit:
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