Influence of inflammation on serum concentration, molecular heterogeneity and drug binding properties of canine alpha–1–acid glycoprotein

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Abstract
The concentration and the heterogeneity of alpha-I-acid glycoprotein (.alpha.-1-AGP) and oxprenolol binding were determined serum of healthy dogs and dogs with inflammatory disease. In inflammation, an increase in the mean .alpha.-1-AGP concentration from 0.47 to 2.85 g/l was accompanied by a reduction in the mean free oxprenolol fraction from 25% to 6%. .alpha.-1-AGP concentration and oxprenolol binding were inversely correlated. The heterogeneity of canine .alpha.-1-AGP remained essentially unchanged in dogs with inflammation and, in both these dogs and the controls, between five and seven forms with different isoelectric points and one single concanavalin A-reactive form were detected. It is concluded that in dogs as in humans, oxprenolol binds to serum .alpha.-1-AGP. Changes in serum binding of oxprenolol during inflammation result from a change in the serum concentration of .alpha.-1-AGP rather than a change of molecular heterogeneity.