Microsomal Aryl Hydrocarbon Hydroxylase Comparison of the Direct, Indirect and Radiometric Assays

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Abstract
The direct fluorometric assay of aryl hydrocarbon hydroxylase was compared to the more commonly used indirect fluorometric and radiometric assays. Although rat hepatic microsomal activities measured by the direct assay were consistently higher than those obtained by the other assays, the relative changes in activity following enzyme induction and/or inhibition were similar. The direct assay provided an accurate and rapid measure of aryl hydrocarbon hydroxylase activity and avoided several problems inherent in the indirect and radiometric assays.