Application of a Recombinant Baculovirus Expressing Hantavirus Nucleocapsid Protein as a Diagnostic Antigen in IFA Test: Cross Reactivities among 3 Serotypes of Hantavirus which Causes Hemorrhagic Fever with Renal Syndrome(HFRS).
- 1 January 1993
- journal article
- Published by Japanese Society of Veterinary Science in The Journal of Veterinary Medical Science
- Vol. 55 (6) , 1047-1050
- https://doi.org/10.1292/jvms.55.1047
Abstract
Recombinant baculovirus-infected insect cells which expressed recombinant protein, analogous to the nucleocapsid protein (NP) of Seoul type hantavirus, strain SR-11 (rNP-SR-Sf9) were applied to the indirect immunofluorescent antibody (IFA) test. The rNP-SR-Sf9 reacted with anti NP MAb clones which recognized strain specific or hantavirus common epitopes in the IFA test. The recombinant antigen was detected by antibodies to 3 serotypes of hantaviruses (Hantaan 76-118, SR-11, Puumala). Antibody titers of a group of experimentally infected mouse and urban rat sera using rNP-SR-Sf9 and strain SR-11-infected Vero cell antigens were mutual correlative. These results indicated that the rNP-SR-Sf9 were an effective and safety substitute for Vero E6 cells in the IFA test for serosurveys of hantavirus infection.Keywords
This publication has 0 references indexed in Scilit: