The effect of prestorage white cell reduction on the function and viability of stored platelet concentrates
- 1 May 1992
- journal article
- Published by Wiley in Transfusion
- Vol. 32 (4) , 334-339
- https://doi.org/10.1046/j.1537-2995.1992.32492263447.x
Abstract
The role of residual donor white cells (WBCs) in producing the storage lesion of platelets used for transfusion was studied. The effect of prestorage WBC reduction on in vitro and in vivo measurements of the quality of stored platelet concentrates (PCs) was examined by using a newly developed WBC‐reduction filter capable of preparing PCs with a mean residual WBC concentration of less than 1 per microL. For in vitro studies, a triplet study design was used, in which WBC‐reduced PCs were matched to standard PCs and to WBC‐enriched PCs obtained from the same donor at the same phlebotomy. Twelve donors were studied. Prestorage WBC reduction resulted in a higher pH and pO2 and a lower pCO2 than in standard PCs. In accord with previous in vitro studies, a significant rise in plasma glycocalicin and lactate dehydrogenase was measured during storage, but the levels were not significantly different in WBC‐ reduced PCs and standard PCs. Platelet aggregation and ATP release in response to graded doses of thrombin was similar in WBC‐reduced and standard PCs. In vivo recovery and survival studies were comparable in WBC‐reduced and standard PCs. Although the residual donor WBC content of PCs has a significant impact on storage pH, pO2, and pCO2, prestorage WBC reduction does not affect platelet structure, function, or viability as assessed by in vitro or in vivo measurements.Keywords
This publication has 34 references indexed in Scilit:
- Peroxidative Events in Stored Platelet Concentrates1Vox Sanguinis, 1989
- Platelet membrane glycoprotein changes during the preparation and storage of platelet concentratesTransfusion, 1988
- Preparation of Leukocyte‐Poor Platelet Concentrates from Buffy CoatsVox Sanguinis, 1987
- Human neutrophil elastase modulates platelet function by limited proteolysis of membrane glycoproteins.Journal of Clinical Investigation, 1985
- Platelet Function of Room Temperature Platelet Concentrates Stored in a New Plastic Material with High Gas PermeabilityVox Sanguinis, 1984
- Importance of White Blood Cells in Platelet Storage1Vox Sanguinis, 1984
- Paired comparison of the in vivo and in vitro results of storage of platelet concentrates in two containersTransfusion, 1984
- Kinetics and Distribution of111Indium-Labeled Platelets in Patients with HomocystinuriaNew England Journal of Medicine, 1982
- Factors Influencing Changes in pH during Storage of Platelet Concentrates at 20–24°C1,2Vox Sanguinis, 1982
- Platelet glycolysis in platelet storage. IV. The effect of supplemental glucose and adenineTransfusion, 1980