Neuroprotective Agents: Is Effective on Toxicity in Glial Cells?
- 7 June 2006
- journal article
- Published by Springer Nature in Cellular and Molecular Neurobiology
- Vol. 27 (2) , 171-177
- https://doi.org/10.1007/s10571-006-9082-4
Abstract
1. Glial cells are the most abundant cell population in the central nervous system. The aim of this study was to examine the effects of melatonin, 7-nitroindazole, and riluzole on glutamate toxicity in primary glial cell culture. 2. Glutamate toxicity was induced by addition of 100 μM glutamate to the cultures, and then 100 μM melatonin, 500 μM 7-nitroindazole, and 10 (M riluzole were administered in different groups. Lactate Dehydrogenase activity and nitrite levels were determined at the 1st, 6th, and 24th h. 3. Melatonin, 7-nitroindazole, and riluzole decrease Lactate Dehydrogenase activity at the 1st, 6th, and 24th h (at all hours, p<0.05). Nitrite levels were decreased by melatonin and riluzole at the 1st, 6th, and 24th h. 4. In this study, we observed that melatonin, 7-nitroindazole, and riluzole are effective as protective agents on glutamate toxicity in mixed glial cells.Keywords
This publication has 23 references indexed in Scilit:
- Guanosine Enhances Glutamate Transport Capacity in Brain Cortical SlicesCellular and Molecular Neurobiology, 2005
- Melatonin attenuates 1-methyl-4-phenylpyridinium-induced PC12 cell deathActa Pharmacologica Sinica, 2005
- Protective effects of neuronal nitric oxide synthase inhibitor in mouse brain against MPTP neurotoxicity: an immunohistological studyEuropean Neuropsychopharmacology, 2004
- Riluzole Enhances Glutamate Uptake in Rat Astrocyte CulturesCellular and Molecular Neurobiology, 2004
- Inhibition of the neuronal isoform of nitric oxide synthase significantly attenuates 1-methyl-4-phenylpyridinium (MPP + ) toxicity in vitroJournal Of Neural Transmission-Parkinsons Disease and Dementia Section, 2002
- The cardiac glycoside ouabain potentiates excitotoxic injury of adult neurons in rat hippocampusNeuroscience Letters, 1995
- The NOS Inhibitor, 7-Nitroindazole, Decreases Focal Infarct Volume but Not the Response to Topical Acetylcholine in Pial VesselsJournal of Cerebral Blood Flow & Metabolism, 1994
- Identification of Elastase as a Secretory Protease from Cultured Rat MicrogliaJournal of Neurochemistry, 1992
- Glial changes following an excitotoxic lesion in the CNS—II. AstrocytesNeuroscience, 1991
- Lactic Dehydrogenase Activity in Blood.Experimental Biology and Medicine, 1955