The Analysis of Putrescine in Plant Samples by Automated Hplc
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 4 (7) , 1269-1280
- https://doi.org/10.1080/01483918108068811
Abstract
The quantitative analysis of putrescine from plant tissue can be achieved using isocratic reversed-phase high performance liquid chromatography (HPLC). After preliminary extraction and clean up involving an ion exchange purification step, the isolated diamines are derivatised with benzoyl chloride for determination by HPLC. Automation of the HPLC step has led to a considerable saving in time for the total analysis. Potential problems associated with the analytical procedure are described.Keywords
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