Characterization of aNeocallimastixpatriciarumxylanase gene and its product
- 1 November 1999
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 45 (11) , 970-974
- https://doi.org/10.1139/w99-092
Abstract
A xylanase gene (xynC) isolated from the anaerobic ruminal fungus Neocallimastix patriciarum was characterized. The gene consists of an N-terminal catalytic domain that exhibited homology to family 11 of glycosyl hydrolases, a C-terminal cellulose binding domain (CBD) and a putative dockerin domain in between. Each domain was linked by a short linker domain rich in proline and alanine. Deletion analysis demonstrated that the CBD was essential for optimal xylanase activity of the enzyme, while the putative dockerin domain may not be required for enzyme function.Key words: xylanase, cellulose binding domain, Neocallimastix patriciarum.Keywords
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