The Serial Passage of Mixtures of Different Strains of Influenza Virus in Embryonated Eggs and in Mice

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Abstract
Data are presented to demonstrate that mixtures of influenza A and B viruses by serial passage in embryonated eggs and in mice persist. Ten-to 11-day-old embryos were employed. Inoculations consisted of 0.15 ml. of allantoic fluid (10-3) containing 50 U. of penicillin. Incubation was for 55-65 hrs. at 35 C. Three to 5 weeks-old mice were infected intranasally with 0.07 ml. of a 2.5% suspension of infected mouse lung. Identification of the viruses present in infected mouse lungs was by means of protection tests. The results would indicate that a combination of more closely related strains might be maintained indefinitely by the usual methods of laboratory cultivation.

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