Shoots were induced on callus derived from sprout sections and petiole slices of an inbred parent line of Brussels sprout (Brassica oleracea L. var gemmifera D.C.). The shoots, when excised and transferred to fresh medium, enlarged and formed roots. These plantlets could be transferred to soil or their number increased by a multiplication process involving the production of new shoots from the dormant lateral buds. Some of the plantlets derived from sprout callus were grown to maturity in the field and their morphology and chromosome number compared to seed grown plants. There were no significant differences in sprout size and stem diameter but there were significant differences in plant shape. None of the plants in the field experiment showed polyploidy. Plants derived from callus possessed an enhanced ability to form callus and redifferentiate when sections from these plants were placed back on to nutrient medium.