In B-cell chronic lymphocytic leukemias, 7q21 translocations lead to overexpression of the CDK6 gene

Open Access
Publisher Website
Google Scholar
Abstract
B-CLL diagnosis was established according to the World Health Organization Classification of Tumours and was characterized by positivity for CD5 and CD23 expression. All the tumor samples were screened, where possible, by Southern blot, fluorescence in situ hybridization (FISH), reverse-transcriptase real-time quantitative polymerase chain reaction (RT-RQ-PCR), and immunoblotting. In 3 patients, involvement of the CDK6 gene was found by at least 2 different methods. The relevant clinical, biologic, and molecular data for these patients are given in Table 1. FISH and Southern blot analysis showed that an interesting common feature was that in all 3 cases the CDK6 gene was rearranged by reciprocal translocations involving either Igκ, Igλ, or IgH locus and not with only the Igκ light chain locus as has previously been reported in other series.2,3