Cell Fractionation on Affinity Columns

Publisher Website
Google Scholar
Abstract
Nonimmune C57BL/6f mouse spleen lymphocytes were fractionated on glass-bead columns coated with keyhole limpet hemocyanin (KLH). Adherent cells conferred enhanced humoral anti-KLH responses on irradiated syngeneic recipients when compared with nonadherent or unfractionated cells. This effect was unaltered by simultaneous transfer of unfractionated nonimmune thymocytes, but it was not observed when cells were fractionated on a column coated with control antigen, when nonviable cells were transferred, and when cells were fractionated in the presence of free KLH. No adjuvant effect of KLH transferred with cells was demonstrable. By autoradiography with 125I-KLH, adherent lymphocytes were enriched in antigen-binding cells (0.24%), relative to nonadherent cells (0.03%,) with an estimated incidence of 0.06% for KLH-binding lymphocytes in nonimmune C57BL/6f mouse spleen.

This publication has 0 references indexed in Scilit: