FLUOROURACIL SYNCHRONIZATION OF HUMAN-BONE MARROW CULTURES - INVITRO INDUCTION OF HIGH-RESOLUTION R-BANDING BY SIMULTANEOUS EXPOSURE TO 5-BROMODEOXYURIDINE HOECHST-33258

  • 1 January 1984
    • journal article
    • research article
    • Vol. 4  (4-5) , 279-282
Abstract
A simple R-banding technique, based on a fluorouracil S-phase block, released by exposure to 5-bromodeoxyuridine and Hoechst 33258 is applied to human bone marrow cells. It is both simple and reliable, plus ensuring an index corresponding to that of the methotrexate method. Due to Hoechst 33258 exposure prior to harvest, chromosome contraction is partly inhibited. Well defined R-banding in elongated prometaphase chromosomes is observed after traditional Giemsa and FPG-staining [and can be used for karyotyping of human leukemic bone marrow cells.].

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