ATPase activity associated with isolated vacuoles of the crassulacean acid metabolism plant Kalanchoë daigremontiana
- 1 October 1984
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 162 (4) , 299-304
- https://doi.org/10.1007/bf00396740
Abstract
A technique is described that allows a relatively rapid and controlled isolation of vacuoles from leaves of the crassulacean acid metabolism (CAM) plant Kalanchoë daigremontiana. The method involves polybase-induced lysis of mesophyllcell protoplasts and isolation of vacuoles on a discontinuous density gradient. ATPase activity is associated with the isolated vacuoles and is not attributable to contamination by cytoplasmic constituents. It is suggested that this ATPase is responsible for the energization of malic-acid accumulation in the vacuole in CAM plants.Keywords
This publication has 31 references indexed in Scilit:
- Characterization of the vacuolar ATPase activity of the crassulacean‐acid‐metabolism plantKalanchoë daigremontianaReceptor modulatingEuropean Journal of Biochemistry, 1984
- ATPase activity associated with vacuoles and tonoplast vesicles isolated from the CAM plant, Kalanchoë daigremontianaPhysiologia Plantarum, 1984
- Adenine-nucleotide levels during crassulacean acid metabolism and the energetics of malate accumulation in Kalanchoë tubifloraPlant Science Letters, 1982
- Energetics of malate accumulation in the vacuoles of Kalanchoë tubiflora cellsFEBS Letters, 1981
- Water-relation Parameters of Individual Mesophyll Cells of the Crassulacean Acid Metabolism Plant Kalanchoë daigremontianaPlant Physiology, 1980
- Photosynthetic studies with mesophyll protoplasts from Notonia grandiflora, a crassulacean acid metabolism plantPhysiologia Plantarum, 1980
- Electrochemical investigation of active malic acid transport at the tonoplast into the vacuoles of the CAM plantKalanchoë daigremontianaThe Journal of Membrane Biology, 1979
- Diurnal changes in the malic acid content of vacuoles isolated from leaves of the crassulacean acid metabolism plant, Sedum telephiumFEBS Letters, 1978
- Polybase induced lysis of yeast spheroplastsArchiv für Mikrobiologie, 1975
- Spectrophotometric characteristics of chlorophylls a and b and their phenophytins in ethanolBiochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, 1965