Stoichiometry of the Cre recombinase bound to theloxrecombining site
- 11 September 1992
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 20 (17) , 4451-4455
- https://doi.org/10.1093/nar/20.17.4451
Abstract
The site-specific recombinase Cre from bacteriophage P1 binds and carries out recombinatlon at a 34 bp lox site. The lox site consists of two 13 bp inverted repeats, separated by an 8 bp spacer region. Both the palindromic nature of the site and the results of footprinting and band shift experiments suggest that a minimum of two Cre molecules bind to a lox site. We report here experiments that demonstrate the absolute stoichiometry of the Cre- lox complex to be one molecule of Cre bound per Inverted repeat, or two molecules per lox site.Keywords
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