Large quantity production with extreme convenience of human SDF‐1α and SDF‐1β by a Sendai virus vector
Open Access
- 20 March 1998
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 425 (1) , 105-111
- https://doi.org/10.1016/s0014-5793(98)00207-5
Abstract
We describe a robust expression of human stromal cell‐derived factor‐1α (SDF‐1α) and SDF‐1β, the members of CXC‐chemokine family, with a novel vector system based upon Sendai virus, a non‐segmented negative strand RNA virus. Recombinant SDF‐1α and SDF‐1β were detected as a major protein species in culture supernatants, reached as high as 10 μg/ml. This remarkable enrichment of the products allowed us to use even the crude supernatants as the source for biological and antiviral assays without further concentration nor purification and will thus greatly facilitate to screen their genetically engineered derivatives.Keywords
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