DEMONSTRATION OF VITAMIN B12ANALOGUES IN HUMAN SERA NOT DETECTED BY MICROBIOLOGICAL ASSAY
- 1 May 1982
- journal article
- research article
- Published by Wiley in British Journal of Haematology
- Vol. 51 (1) , 171-173
- https://doi.org/10.1111/j.1365-2141.1982.tb07301.x
Abstract
Sera were absorbed with polyacrylamide beads to which purified human intrinsic factor was attached. This procedure removed the vitamin B12 analogs which were measured by nicrobiological assay with Lactobacillus leichmannii and Euglena gracilis and in an isotope dilution method using intrinsic factor. Such sera still contained B12 analogs that were assayed in an isotope dilution method using a nonintrinsic factor vitamin B12 binder. Such vitamin B12 analogs make up .apprx. 1/2 of the total vitamin B12 analogs in human serum.This publication has 5 references indexed in Scilit:
- Forms of vitamin B12 in radioisotope dilution assays.Journal of Clinical Pathology, 1981
- Comparison of serum vitamin B12 estimation by saturation analysis with intrinsic factor and with R-protein as binding agents.Journal of Clinical Pathology, 1981
- Evidence That Some Patients with Pernicious Anemia Are Not Recognized by Radiodilution Assay for Cobalamin in SerumNew England Journal of Medicine, 1978
- Cobalamin Analogues Are Present in Human Plasma and Can Mask Cobalamin Deficiency because Current Radioisotope Dilution Assays Are Not Specific for True CobalaminNew England Journal of Medicine, 1978
- The Use of Chicken Serum for Measurement of Serum Vitamin B12Concentration by Radioisotope Dilution: Description of Method and Comparison with Microbiological Assay ResultsBritish Journal of Haematology, 1974